Bacterial Cell Division Inhibitor

We here employed proteinligand docking classical molecular mechanics MM. Target because it is the mostconserved protein in bacteria and archeaInthe bacterial cell division process FtsZ undergoes polymerization in aGTP-dependent mannerforming aZ-ring at the midpoint of the cell.

Bacterial Cell Wall An Overview Sciencedirect Topics

These FtsZ inhibitors impair localization of FtsZ into the Z-ring and inhibit bacterial cell division.

Bacterial cell division inhibitor. Bacterial cell division requires accurate selection of the middle of the cell where the bacterial tubulin homologue FtsZ polymerizes into a ring structure. Here we report the effect of cinnamaldehyde on FtsZ and hence on the cell division apparatus. In Escherichia coli site selection is dependent on MinC MinD and MinE.

Bacterial cell division requires accurate selection of the middle of the cell where the bacterial tubulin homologue FtsZ polymerizes into a ring structure. It recruits other cell division proteins which enable cell constriction and formation of the mesosome and two daughter cells. Cinnamaldehyde is a natural product from spices that inhibits cell separation in Bacillus cereus.

The chlorinated analogue UCM53 inhibits the growth of clinical isolates of antibiotic-resistant Staphylococcus aureusand Enterococcus faecalis. To propose potent inhibitors of FtsZ the binding properties ofFtsZwithvariousderivativesofZantrinZZ3wereinvestigatedatanelectroniclevelusingmolecular simulations. MinC acts with MinD to inhibit division at sites other than the midcell by directly interacting with FtsZ.

5 In addition FtsZ is structurally and functionally homologous to mammalian vtubulin 6 suggesting a positive outcome in the discovery of antibacterial agents as was the case for tubulin inhibitors in cancer therapy. The inhibition of a bacterial cell division protein ﬁlamentous temperature-sensitive Z FtsZ prevents the reproduction of Mycobacteria. Since its pharmacophoric groups have been identified here it could be a lead structure for the design of more stable and potent anti-FtsZ agents as well as a vital tool for understanding the regulatory role of FtsZ in cell division.

This system is known to function in conjunction with a second negative regulatory system the nucleoid. Localized dimerization and nucleoid binding drive gradient formation by the bacterial cell division inhibitor MipZ. This work suggests that although cinnamaldehyde is unstable it could be considered a new source for antibacterial drug development.

Cell division is regulated by FtsZ a prokaryotic homolog of tubulin. 9 Peptidoglycan insertion at the septum is blocked in divin-treated cells and the result is a bacteriostatic effect in Gram-negative and Gram-positive bacteria. MinC acts with MinD to inhibit division at sites other than the midcell by directly interacting with FtsZ.

Coli as a means of properly localizing the septum prior to cell division. The Min System is a mechanism composed of three proteins MinC MinD and MinE used by E. FtsZ assembles into the Z-ring at the site of cell division.

Each component participates in generating a dynamic oscillation of FtsZ protein inhibition between the two bacterial poles to precisely specify the mid-zone of the cell allowing the cell to accurately divide in two. This paper introduces divin a small molecule inhibitor of bacterial cell division that may facilitate mechanistic studies of this process. The former is found to inhibit the GTPase activity of the protein in a noncompetitive.

To propose potent inhibitors of FtsZ the binding properties of FtsZ with various derivatives of Zantrin ZZ3 were investigated at an electronic level using molecular simulations. 9 Divin inhibits late stages of bacterial cell division and produces mother and daughter cells that remain physically fused together and share a common cytoplasm. Divin disrupts the assembly of late division proteins reduces peptidoglycan remodeling at the division site and blocks compartmentalization of the cytoplasm.

In contrast to other division inhibitors divin does not interact with the tubulin homologue FtsZ affect chromosome segregation or activate regulatory mechanisms that inhibit cell division. We recently introduced a new compound divin 1 that was discovered using the second track of antibiotic development mentioned above. In the bacterial cell division process FtsZ undergoes polymerization in a GTPdependent manner forming a Zring at the midpoint of the cell.

Epub 2012 Apr 5. The inhibition of a bacterial cell division protein filamentous temperature-sensitive Z FtsZ prevents the reproduction of Mycobacteria. The results reported here have confirmed that cinnamaldehyde inhibits bacterial cell division protein FtsZ.

In Escherichia coli site selection is dependent on MinC MinD and MinE. Naturally occurring phytochemicals with reported antibacterial activity were screened for their ability to inhibit the bacterial cell division protein Escherichia coli FtsZ. Kiekebusch D1 Michie KA Essen LO Loewe J Thanbichler M.

Among the representative compounds coumarins inhibit the GTPase and polymerization activities of this protein effectively. It recruitsother cell divisionpro-teins which enablecell constriction and formation of the mes-osome and two daughter cells5. Further screening with ten coumarin analogs we identified two promising candidates scopoletin and daphnetin.

Erratum For The Report Treadmilling By Ftsz Filaments Drives Peptidoglycan Synthesis And Bacterial Cell Division By A W Bisson Filho Y P Hsu G R Squyres E Kuru F Wu C Jukes Y Sun